EXPERIMENT

In scientific inquiry, an experiment is a method of investigating causal relationships among variables. An experiment is a cornerstone of the empirical approach to acquiring data about the world and is used in both natural sciences and social sciences. An experiment can be used to help solve practical problems and to support or negate theoretical assumptions. Many hypotheses in sciences such as physics can experiment causality by noting that, until some phenomenon occurs, nothing happens; then when the phenomenon occurs, a second phenomenon is observed. But often in science, this situation is difficult to obtain. For example, in the old joke, someone claims that they are snapping their fingers "to keep the tigers away"; and justifies this behavior by saying "see - its working!" While this "experiment" does not falsify the hypothesis "snapping fingers keeps the tigers away", it does not really support the hypothesis - not snapping your fingers does keep the tigers away as well.

To demonstrate a cause and effect hypothesis, an experiment must often show that, for example, a phenomenon occurs after a certain treatment is given to a subject, and that the phenomenon does not occur in the absence of the treatment. A controlled experiment generally compares the results obtained from an experimental sample against a control sample, which is practically identical to the experimental sample except for the one aspect whose effect is being tested (the independent variable). A good example would be a drug trial. The sample or group receiving the drug would be the experimental one; and the one receiving the placebo would be the control one. In many laboratory experiments it is good practice to have several replicate samples for the test being performed and have both a positive control and a negative control.

The results from replicate samples can often be averaged, or if one of the replicates is obviously inconsistent with the results from the other samples, it can be discarded as being the result of an experimental error (some step of the test procedure may have been mistakenly omitted for that sample). Most often, tests are done in duplicate or triplicate. A positive control is a procedure that is very similar to the actual experimental test but which is known from previous experience to give a positive result. A negative control is known to give a negative result. The positive control confirms that the basic conditions of the experiment were able to produce a positive result, even if none of the actual experimental samples produce a positive result.

The negative control demonstrates the base-line result obtained when a test does not produce a measurable positive result; often the value of the negative control is treated as a "background" value to be subtracted from the test sample results. Sometimes the positive control takes the quadrant of a standard curve.An example that is often used in teaching laboratories is a controlled protein assay. Students might be given a fluid sample containing an unknown (to the student) amount of protein